Scientist - Cell-free Protein Synthesis
Company: SwiftScale Biologics
Location: Oakland
Posted on: May 9, 2022
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Job Description:
SwiftScale Biologics uses our cell-free protein synthesis
platform to enable (1) fast protein therapeutic development through
to clinical batches and (2) synthesis of proteins that are very
difficult to express in cells. Our mission is to use our platform
technology (built from the work of Prof. Matthew DeLisa at Cornell
and Prof. Michael Jewett at Northwestern University) to enable our
partners to advance dozens of novel therapeutics to the clinic
where they can improve the lives of thousands of patients. We are
looking for someone who shares our goal of getting life-saving
therapeutics to patients more quickly than would otherwise be
possible to join our tightly-knit team.In this position, you will
drive the expansion of applications of cell-free technology in the
biopharmaceutical industry by designing and implementing research
and development projects. The work will involve cell-free reaction
and cellular protein synthesis optimization, protein purification,
and protein characterization using SDS-PAGE, UPLC, and ELISA
binding evaluation. You'll also work with our platform development
team to improve our platform to make a wider variety of proteins
and scale-up the cell-free protein synthesis process from the bench
scale to GMP clinical batches.ResponsibilitiesImprove and build
upon our cell-free synthesis, purification, and characterization
platform to deliver new classes of proteins, expand throughput, and
increase speed using synthetic biology, bioprocess engineering, and
metabolic engineering.Design and execute experiments to screen,
characterize, and produce preparative amounts of protein
therapeutic candidates.Perform and optimize cell-free protein
expression of various therapeutic proteins using PCR, protein and
DNA electrophoresis, Western blotting, expression, cell culture
(bacterial).Prepare and validate materials for cell-free protein
expression for use at the bench and pilot scale.Purify proteins at
scales from magnetic beads to AKTA Explorer/AKTA Pilot.Characterize
proteins using UPLC, SDS-PAGE, and ELISA.Design plasmid and genomic
DNA using synthetic biology-based methods.Develop automation
methods to increase cell-free protein synthesis throughput and
robustness.Interpret and present experimental data to colleagues
and company leadership.Prepare reagents for testing and process
samples via automated systems in accordance with the laboratory's
standard operating procedures (SOPs)Qualifications[Required] Direct
experience working with molecular cloning, protein expression,
purification, and characterization (preferably of protein
therapeutics).[Required] MS (+4 years experience) or PhD, in
Bioengineering, Biochemistry, Synthetic Biology, Biological
Engineering, Biomedical Engineering, Biotechnology, Microbiology,
Chemical Engineering, or related fields.Demonstrated ability to
interpret, compile, and present experimental data.Ability to work
in a fast-paced small company environment with a drive to
creatively solve problems around how to speed up protein
therapeutic development and experimental workflows.Availability to
work full-time hours at our Oakland, CA location.High intrinsic
motivation and a desire to learn new techniques.Direct experience
with in vitro transcription-translation or cell-free protein
synthesis methods.[Preferred] Direct experience with automation and
high-throughput workflows.Our work environment is focused on
treating everyone on our team with respect, fostering a
collaborative environment, taking ownership of our work, learning
new skills, and having fun.
Keywords: SwiftScale Biologics, Oakland , Scientist - Cell-free Protein Synthesis, Other , Oakland, California
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